Confirmation of Species Status of Corchorus trilocularis and C. pseudo-olitorius by Differential Chromosome Banding and Isozyme Assay

• Khatun Masuda

  Department of Botany, University of Dhaka

• Alam Sheikh Shamimul

  Department of Botany, University of Dhaka

抄録

Two wild jute species, namely Corchorus trilocularis and C. pseudo-olitorius, were investigated after staining with orcein, CMA and DAPI. In addition, activities of 3 isozymes viz. esterase, acid phosphatase and peroxidase were studied. These 2 spp. were found to possess 2n=14 chromosomes. Satellites were observed only in C. pseudo-olitorius. The staining property of satellites was striking since a pair appeared in orcein, 2 pairs in CMA and none in DAPI-staining. Each species possessed distinct CMA and DAPI banded karyotypes. Moreover, different activities of acid phosphatase, peroxidase and esterase were found in these 2 species. Fluorescent banding and isozyme analysis showed that C. pseudo-olitorius and C. trilocularis are completely different species.

収録刊行物

• ＣＹＴＯＬＯＧＩＡ

  ＣＹＴＯＬＯＧＩＡ 75 (1), 83-88, 2010

  公益財団法人 日本メンデル協会

キーワード

• Fluorescent banding
• Isozyme analysis
• Karyotype, <i>Corchorus</i> spp

詳細情報

• CRID: 1390001204109415168
• NII論文ID: 10026468123
• NII書誌ID: AA00622764
• DOI: 10.1508/cytologia.75.83
• ISSN: 13487019
• ISSN: 00114545
• Web Site: http://www.jstage.jst.go.jp/article/cytologia/75/1/75_1_83/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可