Isolation and Characterization of <i>Ochrobactrum intermedium </i>from Imported Cattle Serologically Diagnosed with Bovine Brucellosis

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  • 血清検査においてブルセラ病と診断された輸入牛から分離された<i>Ochrobactrum intermedium </i>の同定事例
  • 血清検査においてブルセラ病と診断された輸入牛から分離されたOchrobactrum intermediumの同定事例
  • ケッセイ ケンサ ニ オイテ ブルセラビョウ ト シンダン サレタ ユニュウギュウ カラ ブンリ サレタ Ochrobactrum intermedium ノ ドウテイ ジレイ

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Abstract

More than ten cattle were diagnosed with bovine brucellosis in each of the last several years using serological testing during import quarantine. However, Brucella abortus has not been isolated from the samples of the cattle. In 2008, a bacterium biochemically closed to the genus Brucella was isolated from the lung of one of the feeder cattle imported from Australia, which was serologically diagnosed with brucellosis during import quarantine. The bacterium tested positive in a plate agglutination test for Brucella-specific antisera, and tested positive in a PCR assay for Brucella spp. The bacterium was identified as Ochrobactrum intermedium by PCR assay of the recA genes and genetic analysis using 16s rRNA. The PCR assay of recA genes and the growth on SSagar is useful for identifying Ochrobactrum spp. and Brucella spp.

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