Isolation and Characterization of <i>Ochrobactrum intermedium </i>from Imported Cattle Serologically Diagnosed with Bovine Brucellosis
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- YAMAMOTO Kazuaki
- Animal Quarantine Service
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- TAMURA Takashi
- 農林水産省動物検疫所中部空港支所
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- OKAMURA Yukitake
- 農林水産省消費・安全局国際衛生対策室
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- KABAMOTO Ayako
- Animal Quarantine Service
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- MORIWAKI Shunei
- Animal Quarantine Service
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- ETO Mariko
- Animal Quarantine Service
Bibliographic Information
- Other Title
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- 血清検査においてブルセラ病と診断された輸入牛から分離された<i>Ochrobactrum intermedium </i>の同定事例
- 血清検査においてブルセラ病と診断された輸入牛から分離されたOchrobactrum intermediumの同定事例
- ケッセイ ケンサ ニ オイテ ブルセラビョウ ト シンダン サレタ ユニュウギュウ カラ ブンリ サレタ Ochrobactrum intermedium ノ ドウテイ ジレイ
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Abstract
More than ten cattle were diagnosed with bovine brucellosis in each of the last several years using serological testing during import quarantine. However, Brucella abortus has not been isolated from the samples of the cattle. In 2008, a bacterium biochemically closed to the genus Brucella was isolated from the lung of one of the feeder cattle imported from Australia, which was serologically diagnosed with brucellosis during import quarantine. The bacterium tested positive in a plate agglutination test for Brucella-specific antisera, and tested positive in a PCR assay for Brucella spp. The bacterium was identified as Ochrobactrum intermedium by PCR assay of the recA genes and genetic analysis using 16s rRNA. The PCR assay of recA genes and the growth on SSagar is useful for identifying Ochrobactrum spp. and Brucella spp.
Journal
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- Journal of the Japan Veterinary Medical Association
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Journal of the Japan Veterinary Medical Association 63 (8), 615-619, 2010
Japan Veterinary Medical Association
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Details 詳細情報について
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- CRID
- 1390282679690311680
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- NII Article ID
- 10026611567
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- NII Book ID
- AN00191857
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- ISSN
- 21860211
- 04466454
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- NDL BIB ID
- 10807000
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed