Effects of Feeder Cells on the Establishment of ES Cells from Mice and Rats. The Difference between Allogenetic and Xenogenetic Feeders.

• Kawase Eihachiro

  Laboratory of Developmental Biology, Meiji Institute of Health Science, 540 Naruda, Odawara 250-0862, Japan Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, 533 Parnassus Ave., San Francisco, CA 94143, USA

• Yoshimizu Tomomi

  NODAI Research Institute, Tokyo University of Agriculture, 1-1-1 Sakuragaoka Setagaya-Ku, Tokyo 156-8502, Japan Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo-cho, Izumi, Osaka 594-1101, Japan

• Hashimoto Koichiro

  Laboratory of Developmental Biology, Meiji Institute of Health Science, 540 Naruda, Odawara 250-0862, Japan

抄録

The technology of mouse embryonic stem (ES) cells is clearly proving to be a very successful method for producing transgenic mice. Although there has been considerable efforts to establish ES cell lines from other animals, most of all lines obtained, so far, have been derived from mice. Here, we examined effects of allogenetic and xenogenetic feeders on the establishment of ES cells from mice and rats. We first used three types of feeder cells on the establishment of rat ES cells; mouse primary embryonic fibroblsts (M-PEF), rat primary embryonic fibroblasts (R-PEF) and a mouse embryonic fibroblast cell line, SL10. We could gain some rat ES cell lines only on R-PEF. RES-DA1 cell line from DA rat strain, has the same morphology as mouse ES cells, alkaline phosphatase activity and the 4C9 antigen. We, moreover, examined effects of M-PEF and R-PEF feeders on the establishment of mouse ES cells in C57BL/6 mice. The mouse ES cell line could be established only on M-PEF feeders. Both rat and mouse ES cell lines had pluripotenciality. These results suggested that the establishment of ES cell lines is more effective on allogenetic feeder cells than on xenogenetic cells.

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 46 (1), 15-22, 2000

  公益社団法人 日本繁殖生物学会

キーワード

• ES cells
• Rat
• LIF
• Feeder cells
• Allogenetic, Xenogenetic

詳細情報

• CRID: 1390001206332181376
• NII論文ID: 130000055091
• NII論文ID: 10026613116
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.46.15
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 5335400
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5335400
• Web Site: http://www.jstage.jst.go.jp/article/jrd/46/1/46_1_15/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000182209
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可