Vertical Microreactor Stack Consist of Poly-(Tetrafluoroethylene) Microfluidics for Immunoassay

• Ukita Yoshiaki

  Lab. of Adv. Sci. and Technol. for Industory, University of Hyogo Research fellow(PD) of JSPS

• Kondo Saki

  Lab. of Adv. Sci. and Technol. for Industory, University of Hyogo

• Takeo Masahiro

  Carbuncle Bio-Scientech. LLC.

• Negoro Seiji

  Carbuncle Bio-Scientech. LLC.

• Kataoka Chiwa

  Graduate School of Engineering, University of Hyogo

• Utsumi Yuichi

  Lab. of Adv. Sci. and Technol. for Industory, University of Hyogo

Bibliographic Information

Other Title

• 放射光によるフッ素樹脂製多機能フィルターの作製とイムノアッセイチップへの応用
• ホウシャコウ ニ ヨル フッソ ジュシセイ タキノウ フィルター ノ サクセイ ト イムノアッセイチップ エ ノ オウヨウ

Abstract

This paper reports the first application of high-aspect ratio PTFE microstruscute, which fabricated by synchrotron radiation induced photo-evaporation process, to enzyme-linked immunosorvent assay. The advantages of PTFE microstructure for the development of lab-on-a-chip due to the extremely high-aspect ratio microstructure and chemical stability of PTFE is discussed. The results of immunoassay shows the successful detection of analyte (mouse IgG) with detection range with 0-100ng/ml. This result suggests the successful immobilization of antibody (anti-mouse IgG goat antibody) onto the x-ray exposed surface of PTFE microstructue and successful demonstration of antigen-antibody reaction in the PTFE high-aspect ratio microstructure. We also demonstrated the detection of polychlorinated biphenyl (PCB). As the result of demonstration, we successfully detected PCB with ranging analyte concentration of 0.1-10 ng/ml.

Journal

• IEEJ Transactions on Electronics, Information and Systems

  IEEJ Transactions on Electronics, Information and Systems 130 (10), 1756-1761, 2010

  The Institute of Electrical Engineers of Japan

Keywords

• poly-(tetrafluoroethylene)
• synchrotron radiation
• Lab-on-a-chip
• enzyme-linked immunosorbent assay

Details

• CRID: 1390001204608466560
• NII Article ID: 10026694072
• NII Book ID: AN10065950
• DOI: 10.1541/ieejeiss.130.1756
• ISSN: 13488155
• ISSN: 03854221
• NDL BIB ID: 10831939
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10831939
• Web Site: http://www.jstage.jst.go.jp/article/ieejeiss/130/10/130_10_1756/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed