ChMCO1 of Cochliobolus heterostrophus is a new class of metallo-oxidase, playing an important role in DHN-melanization

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  • Saitoh Yoshimoto
    Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Japan
  • Izumitsu Kosuke
    Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Japan
  • Morita Atsushi
    Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Japan
  • Shimizu Kiminori
    Medical Mycology Research Center, Chiba University, Japan
  • Tanaka Chihiro
    Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Japan

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  • ChMCO1 of <i>Cochliobolus heterostrophus</i> is a new class of metallo-oxidase, playing an important role in DHN-melanization

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<p>A metallo-oxidase gene from a phytopathogenic filamentous fungus, Cochliobolus heterostrophus was cloned. Structural prediction of ChMco1 indicated that this protein lacks a transmembrane helix and is soluble, whereas other known fungal metallo-oxidases including Saccharomyces cerevisiae FET3 are localized to the cell membrane. The results of searches in fungal genomic databases and phylogenetic analysis of fungal metallo-oxidases revealed that ChMco1 and its allies are distinct homologues of Fet3 and unique to filamentous ascomycetous species including C. heterostrophus. We performed a functional analysis of ChMCO1 by generating null mutants for the ChMco1 gene. The ChMco1 null (ΔChMco1) mutants clearly had reduced melanization, although they showed normal growth and conidiation. Results also show that ΔChMco1 mutants lost laccase activity. These results suggest that ChMCO1 is a novel class of metallo-oxidase that is necessary for laccase activity and melanization.</p>

収録刊行物

  • Mycoscience

    Mycoscience 51 (5), 327-336, 2010

    一般社団法人 日本菌学会

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