アフラトキシンB₁誘導ラット肝癌K2細胞で過剰発現する新規NC33遺伝子

• 倉部 誠也

  東京理科大学

• 古宮 裕子

  東京理科大学

• 望月 清一

  東京理科大学

• 片桐 久美子

  東京理科大学

• 杉山 晶規

  東京理科大学

• 川崎 靖

  東京理科大学

• 田代 文夫

  東京理科大学

書誌事項

タイトル別名

• Deregulated expression of a novel gene, NC33, in aflatoxin B1-induced rat hepatocellular carcinoma K2 cells

抄録

Aflatoxin B₁ (AFB₁) is known to be one of the most potent hepatocarcinogens and causes hepatocellular carcinomas in various experimental animals. We have previously reported that AFB₁-induced rat hepatocellular carcinoma K2 cells over-express 14-3-3 β and c-myc genes which are the key regulators of in vitro and in vivo growth of K2 cells. Nonetheless, it has been speculated that in addition to these genes, some other genes also implicate in AFB₁ hepatocarcinogenesis, because AFB₁ induces genomic alterations. Here, we report the cloning of cDNA encoding rat nuclear protein NC33 (nuclear coiled-coil protein 33 kDa) as a new candidate responsible for AFB₁ hepatocarcinogenesis. The expression level of NC33 mRNA in K2 cells was extremely higher than that in normal rat liver. The colony forming ability of K2 cells in semi-solid medium as a hallmark of tumor cells in vitro was diminished by the down-regulation of NC33 gene expression. Thus, it is most likely that the deregulated expression of NC33 gene is involved in AFB₁ hepatocarcinogenesis.

収録刊行物

• マイコトキシン

  マイコトキシン 55 (2), 111-115, 2005

  日本マイコトキシン学会

キーワード

• ラット肝癌細胞
• アフラトキシンB₁
• 肝発癌
• 足場非依存的増殖

詳細情報

• CRID: 1390001204783738112
• NII論文ID: 10026891191
• NII書誌ID: AN00334513
• DOI: 10.2520/myco.55.111
• COI: 1:CAS:528:DC%2BD2MXhtV2jtrfN
• ISSN: 18810128
• ISSN: 02851466
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030750545
• Web Site: https://search.jamas.or.jp/link/ui/2007192305
• 本文言語コード: en
• データソース種別:
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