Real-time PCR in Rapid Diagnosis of <i>Aeromonas hydrophila </i>Necrotizing Soft Tissue Infections

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  • KOHAYAGAWA Yoshitaka
    Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital
  • IZUMI Yoko
    Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital
  • USHITA Misuzu
    Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital
  • NIINOU Norio
    Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital
  • KOSHIZAKI Masayuki
    Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital
  • Yuji YAMAMORI
    Department of Emergency and Critical Care Medicine, Shimane Prefectural Central Hospital
  • KANEKO Sakae
    Department of Dermatology, Shimane Prefectural Central Hospital
  • FUKUSHIMA Hiroshi
    Shimane Prefectural Institute of Public Health and Environmental Science

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Other Title
  • リアルタイムPCR を用いた<i>Aeromonas hydrophila </i>壊死性軟部組織感染症の迅速診断
  • 症例 第82回日本感染症学会総会学術講演会座長推薦論文 リアルタイムPCRを用いたAeromonas hydrophila壊死性軟部組織感染症の迅速診断
  • ショウレイ ダイ82カイ ニホン カンセンショウ ガッカイ ソウカイ ガクジュツ コウエンカイ ザチョウ スイセン ロンブン リアルタイム PCR オ モチイタ Aeromonas hydrophila エシセイナンブ ソシキ カンセンショウ ノ ジンソク シンダン

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Abstract

We report a case of rapidly progressive necrotizing soft tissue infection and sepsis followed by a patientʼs death. We suspected Vibrio vulnificus infection because the patientʼs underlying disease was cirrhosis and the course extremely rapid. No microbe had been detected at death. We extracted DNA from a blood culture bottle. SYBR green I real-time PCR was conducted but could not detect V. vulnificus vvh in the DNA sample. Aeromonas hydrophila was cultured and identified in blood and necrotized tissue samples. Real-time PCR was conducted to detect A. hydrophila ahh1, AHCYTOEN and aerA in the DNA sample extracted from the blood culture bottle and an isolated necrotized tissue strain, but only ahh1 was positive. High-mortality in necrotizing soft tissue infections makes it is crucial to quickly detect V. vulnificus and A. hydrophila.We found real-time PCR for vvh, ahh1, AHCYTOEN,andaerA useful in detecting V. vulnificus and A. hydrophila in necrotizing soft tissue infections.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 83 (6), 673-678, 2009

    The Japanese Association for Infectious Diseases

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