Hydrogen Sulfide, a Gaseous Transmitter, Stimulates Proliferation of Interstitial Cells of Cajal via Phosphorylation of AKT Protein Kinase
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- Huang Ying
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University Department of General Surgery, Union Hospital, Fujian Medical University
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- Li Fan
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University
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- Tong Weidong
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University
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- Zhang Anping
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University
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- He Yujun
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University
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- Fu Tao
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University
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- Liu Baohua
- Department of General Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University
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抄録
Interstitial cells of Cajal (ICC) are distributed throughout the gastrointestinal (GI) tract and have important functions in the control of GI motility. Loss of ICC is associated with several GI motility disorders; yet, the mechanisms modulating ICC survival and proliferation are not fully understood. Hydrogen sulfide (H2S) has been reported to be a gaseous transmitter that regulates cellular proliferation. This study aims to establish whether H2S participates in regulation of ICC proliferation. The effect of H2S was studied in primary cultures of ICC, prepared from the mouse small intestine. To determine the extent of ICC proliferation, we used immunofluorescent staining to study alterations in the number of cells expressing c-Kit+ and CD44+, markers for mature ICC. Phosphorylation of Akt was measured by Western blot analysis. Treatment with low concentrations of NaHS (H2S donor, 1-30 μM) showed no apparent toxicity, as judged from cell numbers. Importantly, treatment with NaHS (15 μM) for 24 hours increased the numbers of c-Kit+/CD44+ ICC by 23.3 ± 1.4% (P < 0.05). Moreover, NaHS increased Akt phosphorylation, which was prevented with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 (5 μM). LY294002 also blocked the NaHS-mediated increase in the number of ICC. In addition, H2S enhanced the proliferation of mature ICC in the in vitro culture system used here in a concentration-dependent manner. The present study suggests that H2S may be a critical factor in maintaining ICC numbers and may have a novel, Akt-dependent role in proliferation of mature ICC.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 221 (2), 125-132, 2010
東北ジャーナル刊行会
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詳細情報 詳細情報について
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- CRID
- 1390001204244323072
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- NII論文ID
- 10027084747
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- NII書誌ID
- AA00863920
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- ISSN
- 13493329
- 00408727
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可