Tsukuba-green: A Fluorescent Dye that Emits Green Fluorescence Useful for Live-cell Imaging

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  • Naoko Senda
    Graduate School of Pure and Applied Sciences, University of Tsukuba
  • Yoshihiro Miwa
    Department of Molecular Pharmacology, Institue of Basic Medical Sciences, University of Tsukuba
  • Junko Tanaka
    Center for Tsukuba Advanced Research Alliance, University of Tsukuba
  • Atsuya Momotake
    Graduate School of Pure and Applied Sciences, University of Tsukuba
  • Tatsuo Arai
    Graduate School of Pure and Applied Sciences, University of Tsukuba

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<jats:title>Abstract</jats:title> <jats:p>A new fluorescent dye, 4-ethoxycarbonylmethyl-2-methyl-7-hydroxyquinoline (TG) was obtained unexpectedly from the BiCl3-catalyzed Pechmann condensation. TG emits green fluorescence at 509 nm in water. For live-cell imaging applications, TG derivatives TG1–TG3 were prepared. Under physiological conditions, HEp-2 cells were immediately stained upon addition of a solution of TG3.</jats:p>

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  • Chemistry Letters

    Chemistry Letters 39 (3), 308-310, 2010-02-20

    Oxford University Press (OUP)

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