Simultaneous Determination of Isoniazid, Pyrazinamide, Rifampicin and Acetylisoniazid in Human Plasma by High-Performance Liquid Chromatography
-
- ZHOU Zhifeng
- Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University
-
- CHEN Lingyun
- Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University
-
- LIU Peng
- Department of Orthopedic and Spinal Surgery, Nanfang Hospital, Southern Medical University
-
- SHEN Mei
- Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University
-
- ZOU Fei
- Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University
この論文をさがす
抄録
A sensitive and accurate high-performance liquid-chromatography method was developed and validated for the simultaneous determination of pyrazinamide (PZA), isoniazid (INH), rifampicin (RFP) and acetylisoniazid (AcINH) in human plasma. Separation was performed on a Max-RP C12 column using gradient elution and a flow-rate program. The mobile phase was methanol–acetonitrile–buffer (20 mM of heptanesulfonic acid sodium, pH 2.5) with a ratio of 10:8:82 (v/v/v) at the initial phase. All calibration curves had good linearity (r2 > 0.99) between the test ranges. The intra- and inter-day precision was less than 8.8% in good accuracy (<15%). The limit of detection with a signal-to-noise (S/N) of 3 was 0.014, 0.009, 0.023 and 0.054 μg mL−1 for PZA, AcINH, INH and RFP, respectively. The method was selective, sensitive and reliable, and is a good alternative for routine therapeutic drug monitoring of the four compounds during the treatment of tuberculosis patients.
収録刊行物
-
- Analytical Sciences
-
Analytical Sciences 26 (11), 1133-1138, 2010
社団法人 日本分析化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679237540608
-
- NII論文ID
- 10027315955
-
- NII書誌ID
- AA10500785
-
- ISSN
- 13482246
- 09106340
-
- NDL書誌ID
- 10873074
-
- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I10873074
- https://link.springer.com/content/pdf/10.2116/analsci.26.1133.pdf
- https://link.springer.com/article/10.2116/analsci.26.1133/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/26/11/26_11_1133/_pdf
- https://search.jamas.or.jp/link/ui/2011132300
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可