Evaluation of Once- Versus Twice-Weekly Transvaginal Ultrasound-Guided Follicular Oocyte Aspiration With or Without FSH Stimulation from the Same Cows.
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- GOTO Kazufumi
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- TANIMOTO Yasuyuki
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- FUJII Wataru
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- TANIGUCHI Shinichiro
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- TAKESHITA Kazuhisa
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- YANAGITA Koichi
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- OOKUTSU Syoji
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
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- NAKANISHI Yoshihiko
- Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan
書誌事項
- タイトル別名
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- Evaluation of Once-Versus Twice-Weekly
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抄録
Ultrasound-guided transvaginal follicular oocyte aspiration combined with in vitro maturation/in vitro fertilization/in vitro culture (IVM/IVF/IVC) was used to obtain bovine embryos. The effects of aspiration frequency and FSH stimulation on follicular development, oocyte recovery and embryo development following IVM/IVF/IVC were evaluated. In Experiment 1, transvaginal follicular aspiration was performed once or twice weekly with or without FSH stimulation in the same cows (2 cows). During the 1st session (5 aspirations/2.5 weeks), follicular aspiration was performed twice a week following the administration of FSH for 2 days (a total of 18 mg FSH). During the 2nd session (5 aspirations/2.5 weeks), follicular aspiration was performed twice a week without FSH treatment. During the 3rd session (5 aspirations/5 weeks), follicular aspiration was performed once a week without FSH treatment. During the 4th session (5 aspirations/5 weeks), follicular aspiration was performed once a week following the administration of FSH for 2 days. The follicular fluid containing aspirated oocytes was kept warm (36 C) in the 2nd, 3rd and 4th sessions but it was kept at air temperature (2-8 C, winter) in the 1st session. There were no significant differences in the mean number of follicles ≥ 2 mm in diameter during the 4 treatment sessions. The mean number of oocytes collected from each cow remained constant during the 4 treatment sessions except in the 1st session with one cow. In vitro development of oocytes collected from each cow showed similar rates of development to the morula/blastocyst stages among the last 3 treatment sessions. The lower development rate of oocytes after IVM/IVF/IVC in the 1st session may be due to the cooling of oocytes after aspiration from the ovarian follicles. This was confirmed in Experiment 2 showing that cooling (5 C, 2 h) of oocytes before IVM decreased significantly (P<0.05) the proportions of embryos that cleaved and developed to transferable stage after IVM/IVF/IVC.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 41 (4), 303-309, 1995
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206333451520
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- NII論文ID
- 10027317435
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- NII書誌ID
- AA10936678
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- COI
- 1:CAS:528:DyaK28XisV2ntLk%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 3929004
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可