Gene Expression in Blastocysts Following Direct Injection of DNA Into Testis

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  • OGAWA Shyoso
    Laboratory for Reproduction Engineering, Department of Animal Production, Meiji University, Kanagawa 214, Japan
  • HAYASHI Katsuhiko
    Laboratory for Reproduction Engineering, Department of Animal Production, Meiji University, Kanagawa 214, Japan
  • TADA Norihiro
    Laboratory Animal Center, Pharma Research and Development Division, Hoechst Japan, Limited, Saitama 350-11, Japan
  • SATO Masahiro
    Laboratory Animal Center, Pharma Research and Development Division, Hoechst Japan, Limited, Saitama 350-11, Japan
  • KURIHARA Takashi
    Laboratory of Bacteriology, National Children's Medical Research Center, 3-35-31 Taishido, Setagaya-ku, Tokyo 214, Japan
  • IWAYA Makoto
    Laboratory of Bacteriology, National Children's Medical Research Center, 3-35-31 Taishido, Setagaya-ku, Tokyo 214, Japan

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タイトル別名
  • Gene Expression in Blastocysts Followin

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Liposome-encapsulated plasmid pMT-neo/MT-β-gal DNA containing a neomycin resistance gene and a E. coli β-galactosidase gene was injected through a needle into mature mouse testes bilaterally 3 times at 4-day intervals. Eighty percent of blastocysts derived from eggs fertilized by spermatozoa of male mice injected with the DNA exhibited expression of β-galactosidase gene and the presence of pMT-neo/MT-β-gal DNA. Thus, direct injection of foreign DNA into the testis will provide a novel and convenient tool for production of transgenic animals.

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