ブタ卵子成熟の細胞内制御機構

• 内藤 邦彦

  東京大学医科学研究所獣医学研究部

書誌事項

タイトル別名

• Intracellular Regulation Mechanism of Porcine Oocyte Maturation
• ブタ卵子成熟の細胞内制御機構--1996年度島村賞受賞講演論文
• ブタ ランシ セイジュク ノ サイボウナイ セイギョ キコウ 1996ネンド

抄録

In the present study, a regulation mechanism of porcine oocyte maturation was examined with special focus on maturation promoting factor (MPF), a crucial regulator of cell division cycle composed of catalytic subunit, p34^cdc2 and regulatory subunit, cyclin B, and Mitogen-activated protein kinase (MAPK). At first, an in vitro maturation system using porcine follicular fluid as maturation medium was established in order to mature the oocytes physiologically normal. The oocytes in the germinal vesicle stage (GV) had a significant amount of p34^cdc2 and MAPK, which concentrations were not changed throughout maturation. An extremely small amount of cyclin B was present in GV oocytes and cyclin B was not synthesised during GV. The extremely small amount of cyclin B associated p34^cdc2 was inactivated by tyrosine phosphorylation and MAPK is also inactive during GV. Germinal vesicle breakdown (GVBD) was induced by tyrosine dephosphorylation of p34^cdc2. After GVBD, cyclin B synthesis was started and the amount of cyclin B associated p34^cdc2 and MPF activity were increased dramatically. MAPK was also activated by phosphorylation and maintained the high activity until the second metaphase (Met2). MPF activity was decreased transiently after the first metaphase (Merl) then reactivated at Met2 without tyrosine dephosphorylation. The amount of cyclin B associated p34^cdc2 in Met2 was greater than that in Metl and most of them were stored as pre-MPF. These results suggest that the regulation of porcine oocyte maturation is essentially similar with other reported species but have some modifications specific for porcine oocytes.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 42 (6), j101-j110, 1996

  公益社団法人 日本繁殖生物学会

キーワード

• MPF
• p34^cdc2
• Cyclin B
• MAPK
• Oocyte maturation

詳細情報

• CRID: 1390001206336294784
• NII論文ID: 10027319523
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.96-426j101
• COI: 1:CAS:528:DyaK2sXks1Wqur8%3D
• ISSN: 13484400
• ISSN: 09168818
• ISSN: http://id.crossref.org/issn/09168818
• NDL書誌ID: 4163811
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4163811
• Web Site: http://www.jstage.jst.go.jp/article/jrd/42/6/42_96-426j101/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1997165437
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可