卵巣を摘出したSCIDマウスへのウシ二次卵胞の異種移植

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献3件 参考文献50件
  • SENBON Shoichiro
    Graduate School of Science and Technology, Kobe University
  • OTA Atsushi
    Graduate School of Science and Technology, Kobe University
  • TACHIBANA Masao
    Graduate School of Science and Technology, Kobe University
  • MIYANO Takashi
    Laboratory of Reproductive Biology and Biotechnology, Faculty of Agriculture, Kobe University

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タイトル別名
  • Xenografting of Bovine Secondary Follicles into Ovariectomized Female Severe Combined Immunodeficient Mice

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Xenografting of ovarian tissue into immunodeficient mice has been used as a model to study the dynamics of follicular development and provides an alternative method for the production of mature oocytes. In a previous experiment, we demonstrated that xenografted bovine secondary follicles developed to the antral stage in severe combined immunodeficient (SCID) mice. In the present study, we examined the development of bovine secondary follicles (140-190 μm in diameter) grafted into ovariectomized mice in comparison with intact female mice as a control. At 4 weeks after grafting, several antral follicles ranging from 350 to 550 μm (457.6 ± 50.8 μm) in diameter were found in the control mice, while a single large (larger than 2.5 mm) antral follicle and other small follicles were observed in every ovariectomized mouse. At 6 weeks after grafting, the mean diameter of morphologically normal follicles had further increased in the control group (591.8 ± 132.0 μm). In ovariectomized mice, however, the mean diameter of follicles decreased (4 weeks: 864.2 ± 988.2 μm; 6 weeks: 496.5 ± 137.6 μm), since the single large antral follicle observed at 4 weeks had degenerated by 6 weeks. In control mice, more than 70% of follicles were morphologically normal and formed an antrum, and most of the follicles contained morphologically normal oocytes which grew to 122.5 ± 2.2 μm. In ovariectomized mice, morphologically normal oocytes also grew larger than before grafting, but their survival rate was significantly lower than that in control mice. These results suggest that ovariectomy of host mice alters the developmental pattern of xenografted bovine secondary follicles to accelerate a single follicle to develop in the graft.<br>

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