メンブレンインサートを用いたマウス二次卵胞の体外培養と培養卵胞から排卵された卵子の発生能

• ADAM Ahmed Abdel Gadir

  Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

• TAKAHASHI Yoshiyuki

  Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

• KATAGIRI Seiji

  Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

• NAGANO Masashi

  Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

書誌事項

タイトル別名

• In Vitro Culture of Mouse Preantral Follicles Using Membrane Inserts and Developmental Competence of In Vitro Ovulated Oocytes

抄録

To develop a reliable follicle culture system, mouse preantral follicles 150-200 μm in diameter were cultured individually for 5 or 6 days in membrane inserts or in droplets, and then induced to ovulate with hCG (Experiment 1). The nuclear maturation and developmental competence of the oocytes that ovulated from the follicles cultured in inserts were determined (Experiment 2). There was no significant difference between the two culture systems in the survival rate (83 and 77%). However, follicles cultured in inserts showed a higher ovulation rate (63%) than those cultured in droplets (39%, P<0.05). About 80% of the oocytes that ovulated from the follicles cultured in inserts were at the metaphase II stage. After in vitro fertilization, 75 and 48% of in vitro ovulated oocytes cleaved and developed into blastocysts, respectively. These results demonstrate that the insert culture system is superior to the droplet culture system in terms of follicular growth and ovulation, and can be used to investigate the growth and ovulation of follicles in vitro.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 50 (5), 579-586, 2004

  公益社団法人 日本繁殖生物学会

キーワード

• Culture system
• <i>In vitro</i> ovulation
• Mouse preantral follicle
• Oocyte maturation

詳細情報

• CRID: 1390001206337100800
• NII論文ID: 10027323486
• NII論文ID: 220000094692
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.50.579
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 7116783
• PubMed: 15514465
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030701492
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7116783
• Web Site: http://www.jstage.jst.go.jp/article/jrd/50/5/50_5_579/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005030547
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可