Developmental Competence of HMC Derived Bovine Cloned Embryos Obtained from Somatic Cell Nuclear Transfer of Adult Fibroblasts and Granulosa Cells

• BHOJWANI Sanjay

  Department of Reproductive Biology, Research Institute for the Biology of Farm Animals

• VAJTA Gabor

  Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences

• CALLESEN Henrik

  Section of Reproductive Biology, Department of Animal Breeding and Genetics, Danish Institute of Agricultural Sciences

• ROSCHLAU Knut

  Rinder Produktion Niedersachsen

• KUWER Andreas

  Rinder Produktion Niedersachsen

• BECKER Frank

  Department of Reproductive Biology, Research Institute for the Biology of Farm Animals

• ALM Hannelore

  Department of Reproductive Biology, Research Institute for the Biology of Farm Animals

• TORNER Helmut

  Department of Reproductive Biology, Research Institute for the Biology of Farm Animals

• KANITZ Wilhelm

  Department of Reproductive Biology, Research Institute for the Biology of Farm Animals

• POEHLAND Ralf

  Department of Reproductive Biology, Research Institute for the Biology of Farm Animals

抄録

To enable us to handle a large number of oocytes at a given time and to have an increased throughput of cloned embryos, we attempted the Handmade cloning (HMC^TM) technique, a zona-free method of bovine somatic cell nuclear transfer. Our objective was to study the developmental competence of the HMC^TM derived embryos obtained using different types of somatic cells. A total of 6,874 cumulus-oocyte-complexes were used with either 7th or 11th passage fibroblasts (1st and 2nd groups, respectively), which were prepared from male animals, or granulosa cells (3rd group) as nuclei donors. The average cleavage rate was 65%, accompanied by a blastocyst rate of just 2% for the cleaved products and 5% for the > 8-cell embryos, and there was no significant difference between the three groups. Out of 27 blastocysts recovered, 22 blastocysts were transferred to 22 recipients, resulting in two pregnancies. One pregnancy was lost after the fourth week while the other progressed to full term with the birth of a male calf. This first successful cloning of a male calf with the HMC^TM technique in Europe indicates the successful adoption and establishment of this technique in our laboratory, and that this technique can be successful in producing viable embryos.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 51 (4), 465-475, 2005

  公益社団法人 日本繁殖生物学会

キーワード

• Bovine
• Fibroblast
• Granulose cell
• Somatic cell nuclear transfer

詳細情報

• CRID: 1390282681313888896
• NII論文ID: 10027325387
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.17025
• COI: 1:STN:280:DC%2BD2Mvnt12rsA%3D%3D
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 7412283
• PubMed: 16141646
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7412283
• Web Site: http://www.jstage.jst.go.jp/article/jrd/51/4/51_4_465/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006089117
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可