Prevention of ochratoxin A‐induced neural tube defects by folic acid in the genetic polydactyly/arhinencephaly mouse,<i>Pdn/Pdn</i>

• Ryu‐ichi Katagiri

• Maho Kurome

• Yuko Teshima

• Etsuko Ueta

• Ichiro Naruse

書誌事項

タイトル別名

• Prevention of ochratoxin A-induced neural tube defects by folic acid in the genetic polydactyly/arhinencephaly mouse, Pdn/Pdn

抄録

ABSTRACT  The gene responsible for the polydactyly/arhinencephaly (Pdn/Pdn) mouse, which exhibits polysyndactyly and arhinencephaly and has a 13.2% risk of neural tube defects (NTD), has been identified as Gli3. Ochratoxin A (OTA) is a teratogen causing NTD in mice. When Pdn/Pdn embryos were exposed to 2 mg/kg of OTA on day 7.5, the incidence of NTD in Pdn/Pdn fetuses increased to 51.6%. Pre-treatment with folinic acid (FA), metabolically the most active form of folic acid, before OTA-treatment decreased the incidence of NTD to 20.8%. We investigated the effect of OTA and FA on gene expression in day 9 embryos using whole-mount in situ hybridization and real-time PCR. Over-expression of Fgf8 was observed at the anterior neural ridge (ANR) in the non-treated Pdn/Pdn. Over-expression at the ANR expanded in the OTA-treated Pdn/Pdn, and it was ameliorated by pretreatment with FA. Emx2 signal was observed in the dorsal forebrain in the non-treated +/+, but disappeared in the OTA-treated +/+, and was recovered by FA. The Emx2 signal was pale and the expression amount was depressed in the non-treated and OTA-treated Pdn/Pdn embryos. It was suggested that down-regulation of Gli3 induced the over-expression of Fgf8 at the ANR, that OTA treatment accelerated the over-expression, and that pretreatment with FA ameliorated the OTA-induced over-expression of Fgf8 in the Pdn/Pdn. It was also suggested that down-regulation of Gli3 induced the down-regulation of Emx2 in the Pdn/Pdn. It was further speculated that the over-expression of Fgf8 at the ANR and down-regulation of Emx2 in the dorsal forebrain may contribute to NTD induction.

収録刊行物

• 日本先天異常学会会報

  日本先天異常学会会報 47 (3), 90-96, 2007

  日本先天異常学会

キーワード

• Developmental Biology
• Embryology
• General Medicine
• Pediatrics, Perinatology and Child Health

詳細情報

• CRID: 1390570475138923008
• NII論文ID: 10027386489
• NII書誌ID: AN10066760
• DOI: 10.24540/cgafa.47.3_90
• DOI: 10.1111/j.1741-4520.2007.00152.x
• ISSN: 24331503
• ISSN: 17414520
• ISSN: 00372285
• ISSN: 09143505
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1741-4520.2007.00152.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1741-4520.2007.00152.x
• Web Site: https://search.jamas.or.jp/link/ui/2008095084
• 本文言語コード: en
• データソース種別:
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