Characterization of Theanine-Forming Enzyme from<i>Methylovorus mays</i>No. 9 in Respect to Utilization of Theanine Production

• YAMAMOTO Sachiko

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• WAKAYAMA Mamoru

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• TACHIKI Takashi

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

書誌事項

タイトル別名

• Characterization of Theanine-Forming Enzyme from Methylovorus mays No. 9 in Respect to Utilization of Theanine Production

抄録

For development of theanine production from glutamic acid and ethylamine by coupling yeast sugar fermentation as an ATP-regenerating system, several strains were selected from among about 200 methylamine- and/or methanol-assimilating bacteria depending on the theanine-forming activity of their permeated cells. The amount of theanine formed by the cells of the selected strains was much larger than that by the cells of Escherichia coli AD494 (DE3) expressing Pseudomonas taetrolens Y-30 glutamine synthetase (GS), which has been found to be a usable enzyme for theanine production. A GS-like enzyme responsible for the theanine-forming reaction was obtained from an obligate methylotroph isolate, Methylovorus mays No. 9. The enzyme was induced by methylamine in the culture medium. A molecular mass of 410–470 kDa was obtained by gel filtration of the enzyme, and 51 kDa by SDS–PAGE analysis. The enzyme showed high activity toward methylamine rather than ammonia, which indicates that it is similar to known γ-glutamylmethylamide synthetase. The isolated enzyme also had high reactivity to ethylamine in a neutral pH range, and formed theanine from glutamic acid and ethylamine in a reaction mixture containing a yeast sugar fermentation system for ATP-regeneration.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (2), 545-552, 2007

  公益社団法人 日本農芸化学会

キーワード

• theanine-forming enzyme
• <I>Methylovorus mays</I> No. 9
• coupled fermentation with energy transfer

詳細情報

• CRID: 1390282681453916032
• NII論文ID: 10027511935
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60590
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8674653
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8674653
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60590
• 本文言語コード: en
• データソース種別:
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