Hypocholesterolemic mechanism of Chlorella: Chlorella and its indigestible fraction enhance hepatic cholesterol catabolism through up-regulation of cholesterol 7α-hydroxylase in rats

• SHIBATA Shinya

  Yakult Central Institute for Microbiological Research

• HAYAKAWA Kazuhito

  Yakult Central Institute for Microbiological Research

• EGASHIRA Yukari

  Graduate School of Science and Technology, Chiba University

• SANADA Hiroo

  Graduate School of Science and Technology, Chiba University

書誌事項

タイトル別名

• Hypocholesterolemic Mechanism of Chlorella: Chlorella and Its Indigestible Fraction Enhance Hepatic Cholesterol Catabolism through Up-Regulation of Cholesterol 7.ALPHA.-Hydroxylase in Rats
• Hypocholesterolemic mechanism of Chlorella Chlorella and its indigestible fraction enhance hepatic cholesterol catabolism through up regulation of cholesterol 7アルファ hydroxylase in rats
• Hypocholesterolemic Mechanism of<i>Chlorella</i>:<i>Chlorella</i>and Its Indigestible Fraction Enhance Hepatic Cholesterol Catabolism through Up-Regulation of Cholesterol 7α-Hydroxylase in Rats

抄録

Chlorella powder (CP) has a hypocholesterolemic effect and high bile acid-binding capacity; however, its effects on hepatic cholesterol metabolism are still unclear. In the present study, male Wistar rats were divided into four groups and fed a high sucrose + 10% lard diet (H), an H + 10% CP diet (H+CP), an H + 0.5% cholesterol + 0.25% sodium cholate diet (C), or a C + 10% CP diet (C+CP) for 2 weeks. CP decreased serum and liver cholesterol levels significantly in rats fed C-based diets, but did not affect these parameters in rats fed H-based diets. CP increased the hepatic mRNA level and activity of cholesterol 7α-hydroxylase (CYP7A1). CP increased hepatic HMG-CoA reductase (HMGR) activity in the rats fed H-based diets, but not in rats fed C-based diets. CP did not affect hepatic mRNA levels of sterol 27-hydroxylase, HMGR, low-density lipoprotein (LDL) receptor, scavenger receptor class B1, ATP-binding cassette (ABC) A1, ABCG5, or ABCB11. Furthermore, the effect of a 3.08% Chlorella indigestible fraction (CIF, corresponding to 10% CP) on hepatic cholesterol metabolism was determined using the same animal models. CIF also decreased serum and liver cholesterol levels significantly in rats fed C-based diets. CIF increased hepatic CYP7A1 mRNA levels. These results suggest that the hypocholesterolemic effect of CP involves enhancement of cholesterol catabolism through up-regulation of hepatic CYP7A1 expression and that CIF contributes to the hypocholesterolemic effect.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (4), 916-925, 2007

  公益社団法人 日本農芸化学会

キーワード

• <I>Chlorella</I>
• cholesterol
• bile acids
• cholesterol 7α-hydroxylase (CYP7A1)

詳細情報

• CRID: 1390001206480547712
• NII論文ID: 10027513400
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60566
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8714978
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8714978
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60566
• Web Site: https://search.jamas.or.jp/link/ui/2007258240
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可