Zerumbone Suppresses Phorbol Ester-Induced Expression of Multiple Scavenger Receptor Genes in THP-1 Human Monocytic Cells

• EGUCHI Ai

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• KANEKO Yuki

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• MURAKAMI Akira

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

• OHIGASHI Hajime

  Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University

抄録

Unregulated uptake of oxidized low-density lipoproteins (ox-LDL) via macrophage scavenger receptors (SRs), such as lectin-like ox-LDL receptor-1 (LOX-1), is a key event in atherosclerosis. In the present study, we used differentiated Caco-2 cells as a model of the human small intestine to evaluate the suppressive effects of 16 traditional food items selected from Okinawa on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced LOX-1 mRNA expression in THP-1 human monocyte-like cells. Three Zingiberaceae plants, Curcuma aromatica Salisbury, Curcuma longa L., and Zingiber zerumbet Smith, markedly suppressed that expression. When added to the apical sides of Caco-2 monolayers, zerumbone, a sesquiterpene from Z. zerumbet Smith, was found to permeate into the basolateral medium as an intact structure in a time-dependent manner. α-Humulene, a structural analog of zerumbone lacking the α,β-unsaturated carbonyl group, did not suppress LOX-1 mRNA expression, indicating that its electrophilic moiety might play pivotal roles in its activities. Further, zerumbone attenuated the expression of SR-A, SR-PSOX, and CD36, but not that of CD68 or CLA-1, leading to a blockade of DiI-acLDL uptake, while it also inhibited the transcriptional activities of activator protein-1 and nuclear factor-κB. Together, our results indicate that zerumbone is a potential phytochemical for regulating atherosclerosis with reasonable action mechanisms.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (4), 935-945, 2007

  公益社団法人 日本農芸化学会

キーワード

• oxidized low-density lipoprotein (ox-LDL)
• scavenger receptor
• lectin-like ox-LDL receptor-1 (LOX-1)
• zerumbone
• Caco-2

詳細情報

• CRID: 1390001206480571136
• NII論文ID: 10027513468
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60596
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8714993
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8714993
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60596
• Web Site: https://search.jamas.or.jp/link/ui/2007258241
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可