Purification and Characterization of Two Alkaline, Thermotolerant .ALPHA.-Amylases from Bacillus halodurans 38C-2-1 and Expression of the Cloned Gene in Escherichia coli

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  • MURAKAMI Shuichiro
    Laboratory of Applied Microbiology, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • NISHIMOTO Haruka
    Laboratory of Applied Microbiology, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • TOYAMA Yosuke
    Laboratory of Applied Microbiology, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • SHIMAMOTO Etsuko
    Laboratory of Applied Microbiology, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • TAKENAKA Shinji
    Laboratory of Applied Microbiology, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University
  • KAULPIBOON Jarunee
    Department of Pre-Clinical Science (Biochemistry), Faculty of Medicine, Thammasat University
  • PROUSOONTORN Manchumas
    Cyclodextrin and Starch Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University
  • LIMPASENI Tipaporn
    Cyclodextrin and Starch Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University
  • PONGSAWASDI Piamsook
    Cyclodextrin and Starch Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University
  • AOKI Kenji
    Laboratory of Applied Microbiology, Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University

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Other Title
  • Purification and characterization of two alkaline, thermotolerant α-amylases from Bacillus halodurans 38C-2-1 and expression of the cloned gene in Escherichia coli
  • Purification and characterization of two alkaline thermotolerant a amylases from Bacillus halodurans 38C 2 1 and expression of the cloned gene in Escherichia coli
  • Purification and Characterization of Two Alkaline, Thermotolerant α-Amylases from<i>Bacillus halodurans</i>38C-2-1 and Expression of the Cloned Gene in<i>Escherichia coli</i>

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Abstract

A newly isolated strain, 38C-2-1, produced alkaline and thermotolerant α-amylases and was identified as Bacillus halodurans. The enzymes were purified to homogeneity and named α-amylase I and II. These showed molecular masses of 105 and 75 kDa respectively and showed maximal activities at 50–60 °C and pH 10–11, and 42 and 38% relative activities at 30 °C. These results indicate that the enzymes are thermotolerant. The enzyme activity was not inhibited by a surfactant or a bleaching reagent used in detergents. A gene encoding α-amylase I was cloned and named amyI. Production of AmyI with a signal peptide repressed the growth of an Escherichia coli transformant. When enzyme production was induced by the addition of isopropyl β-D(−)-thiogalactopyranoside in the late exponential growth phase, the highest enzyme yield was observed. It was 45-fold that of the parent strain 38C-2-1.

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