Sterol Biosynthesis by a Prokaryote: First<i>in Vitro</i>Identification of the Genes Encoding Squalene Epoxidase and Lanosterol Synthase from<i>Methylococcus capsulatus</i>

• NAKANO Chiaki

  Graduate School of Science and Technology, Niigata University

• MOTEGI Akihiro

  Graduate School of Science and Technology, Niigata University

• SATO Tsutomu

  Graduate School of Science and Technology, Niigata University Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• ONODERA Masayuki

  Department of Applied Chemistry and Biotechnology, Niigata Institute of Technology

• HOSHINO Tsutomu

  Graduate School of Science and Technology, Niigata University Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

書誌事項

タイトル別名

• Sterol Biosynthesis by a Prokaryote: First in Vitro Identification of the Genes Encoding Squalene Epoxidase and Lanosterol Synthase from Methylococcus capsulatus

抄録

Sterol biosynthesis by prokaryotic organisms is very rare. Squalene epoxidase and lanosterol synthase are prerequisite to cyclic sterol biosynthesis. These two enzymes, from the methanotrophic bacterium Methylococcus capsulatus, were functionally expressed in Escherichia coli. Structural analyses of the enzymatic products indicated that the reactions proceeded in a complete regio- and stereospecific fashion to afford (3S)-2,3-oxidosqualene from squalene and lanosterol from (3S)-2,3-oxidosqualene, in full accordance with those of eukaryotes. However, our result obtained with the putative lanosterol synthase was inconsistent with a previous report that the prokaryote accepts both (3R)- and (3S)-2,3-oxidosqualenes to afford 3-epi-lanosterol and lanosterol, respectively. This is the first report demonstrating the existence of the genes encoding squalene epoxidase and lanosterol synthase in prokaryotes by establishing the enzyme activities. The evolutionary aspect of prokaryotic squalene epoxidase and lanosterol synthase is discussed.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (10), 2543-2550, 2007

  公益社団法人 日本農芸化学会

キーワード

• squalene
• oxidosqualene
• lanosterol
• hopene
• <I>Methylococcus capsulatus</I>

詳細情報

• CRID: 1390001206479684608
• NII論文ID: 10027520168
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70331
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8980826
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8980826
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70331
• 本文言語コード: en
• データソース種別:
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