High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization

• TAKAHASHI Saori

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center

• HATA Keishi

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center

• KIKUCHI Ken-Ichi

  Department of Materials-Process Engineering and Applied Chemistry for the Environment, Akita University

• GOTOH Takeshi

  Department of Materials-Process Engineering and Applied Chemistry for the Environment, Akita University

抄録

Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 71 (10), 2610-2613, 2007

  公益社団法人 日本農芸化学会

キーワード

• renin
• prorenin
• expression
• baculovirus
• Sf-9 cells

詳細情報

• CRID: 1390282681456279936
• NII論文ID: 10027520445
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70416
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 8981086
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8981086
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70416
• Web Site: https://search.jamas.or.jp/link/ui/2008115962
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可