Gene Cloning and Biochemical Characterization of the BMP-2 of<i>Pinctada fucata</i>

• MIYASHITA Tomoyuki

  Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

• HANASHITA Takuo

  Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

• TORIYAMA Michinori

  Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

• TAKAGI Ryousuke

  Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

• AKASHIKA Tamaki

  Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

• HIGASHIKUBO Nobuto

  Department of Genetic Engineering, Faculty of Biology-Oriented Science and Technology, Kinki University

書誌事項

タイトル別名

• Gene Cloning and Biochemical Characterization of the BMP-2 of Pinctada fucata

抄録

The bone morphogenetic proteins (BMPs) constitute a subfamily of the transforming growth factor type beta (TGF-β) supergene family. BMP-2 plays an important role not only in osteoblast differentiation but also in pattern formation during development. To determine the function of BMP-2 in Pinctada fucata development and hard tissue formation, we isolated a BMP-2 genomic DNA clone and the BMP-2 cDNA. The deduced BMP-2 sequence consisted of 447 amino acids. The BMP-2 gene was composed of three exons. The C-terminal portion (149 amino acids) had 86% and 66% identity to the Crassostrea gigas and the human BMP-2 sequence respectively. The 5′ flanking promoter region contained putative glioma transcription factor (Gli) and retinoic acid receptor (RAR) responding elements. The BMP-2 gene was expressed strongly in the inner part of the mantle tissue, corresponding to the nacreous aragonite shell layer. This finding suggests that BMP-2 has a key role in nacreous layer formation.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (1), 37-47, 2008

  公益社団法人 日本農芸化学会

キーワード

• <I>Pinctada fucata</I>
• transforming growth factor type beta (TGF-β)
• gene structure
• cDNA cloning
• biomineralization

詳細情報

• CRID: 1390282681454826880
• NII論文ID: 10027522787
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70302
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9364941
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9364941
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70302
• 本文言語コード: en
• データソース種別:
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