Construction of a Binary Vector for Knockout and Expression Analysis of Rice Blast Fungus Genes

• SAITOH Ken-ichiro

  Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)

• NISHIMURA Marie

  Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)

• KUBO Yasuyuki

  Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University

• HAYASHI Nagao

  Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)

• MINAMI Eiichi

  Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)

• NISHIZAWA Yoko

  Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)

抄録

We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (5), 1380-1383, 2008

  公益社団法人 日本農芸化学会

キーワード

• gene knockout
• green fluorescent protein (GFP)-tagging
• gene expression imaging
• <I>Agrobacterium</I>-mediated transformation
• <I>Magnaporthe grisea</I>

詳細情報

• CRID: 1390001206478725376
• NII論文ID: 10027528215
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70834
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9518974
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9518974
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70834
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可