Ingested .BETA.-Carotene Enhances Glutathione Level and up-Regulates the Activity of Cysteine Cathepsin in Murine Splenocytes

• TAKEDA Saki

  Department of Food Science, Graduate School of Nutrition and Biosciences, The University of Tokushima

• BANDO Noriko

  Department of Food Science, Graduate School of Nutrition and Biosciences, The University of Tokushima

• YAMANISHI Rintaro

  Department of Food Science, Graduate School of Nutrition and Biosciences, The University of Tokushima

Bibliographic Information

Other Title

• Ingested β-carotene enhances glutathione level and up-regulates the activity of cysteine cathepsin in murine splenocytes
• Ingested v carotene enhances glutathione level and up regulates the activity of cysteine cathepsin in murine splenocytes

Abstract

To elucidate health benefits of β-carotene, especially on immunity, we measured redox-related indices in spleen cells from BALB/c mice supplemented with various amounts of β-carotene. In mice supplemented with β-carotene in their diet, glutathione, an intracellular anti-oxidation agent, increased in their splenocytes. This change was highly correlated with the accumulation of β-carotene, but not with that of retinol. The increase in glutathione was accompanied by an increase in mRNA for γ-glutamylcysteine synthetase, a rate-limiting enzyme for glutathione synthesis. The higher the glutathione content was in the spleen cells, the higher the activity of cysteine cathepsin became in crude antigen-presenting cells contained in the spleen. These data suggest that accumulated β-carotene in splenocytes, without being metabolized, caused an increase in the intracellular glutathione level, thereby anti-oxidatively supporting the activity of redox-sensitive lysosomal protease, which is involved in antigen-presentation.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 72 (6), 1595-1600, 2008

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• β-carotene
• glutathione synthesis
• cysteine cathepsin
• murine splenocytes
• antigen-presenting cell

Details

• CRID: 1390282681455408896
• NII Article ID: 10027529039
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.80102
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 9566798
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9566798
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80102
• Web Site: https://search.jamas.or.jp/link/ui/2009014075
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed