Improvement of<scp>L</scp>-Lysine Production by<i>Methylophilus methylotrophus</i>from Methanol<i>via</i>the Entner-Doudoroff Pathway, Originating in<i>Escherichia coli</i>

• ISHIKAWA Kohei

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc. Graduate School of Agriculture, Hokkaido University

• GUNJI Yoshiya

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• YASUEDA Hisashi

  Life Science Laboratories, Ajinomoto Co., Inc.

• ASANO Kozo

  Graduate School of Agriculture, Hokkaido University

書誌事項

タイトル別名

• Improvement of L-Lysine Production by Methylophilus methylotrophus from Methanol via the Entner-Doudoroff Pathway, Originating in Escherichia coli

抄録

To improve the amino acid production by metabolic engineering, eliminating the pathway bottleneck is known to be very effective. The metabolic response of Methylophilus methylotrophus upon the addition of glucose and of pyruvate was investigated in batch cultivation. We found that the supply of pyruvate is a bottleneck in L-lysine production in M. methylotrophus from methanol as carbon source. M. methylotrophus has a ribulose monophosphate (RuMP) pathway for methanol assimilation, and consequently synthesized fructose-6-phosphate is metabolized to pyruvate via the Entner-Doudoroff (ED) pathway, and the ED pathway is thought to be the main pathway for pyruvate supply. An L-lysine producer of M. methylotrophus with an enhanced ED pathway was constructed by the introduction of the E. coli edd-eda operon encoding the enzyme involving the ED pathway. In this strain, the overall enzymatic activity of ED pathway, which is estimated by measuring the activities of 6-phosphogluconate dehydrogenase plus 2-keto-3-deoxy-6-phosphogluconate aldolase, was about 20 times higher than in the parent. This strain produced 1.2 times more L-lysine than the parent producer. Perhaps, then, the supply of pyruvate was a bottleneck in L-lysine production in the L-lysine producer of M. methylotrophus.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (10), 2535-2542, 2008

  公益社団法人 日本農芸化学会

キーワード

• <small>L</small>-lysine production
• <I>edd-eda</I> (ED)
• methanol
• <I>Methylophilus methylotrophus</I>

詳細情報

• CRID: 1390001206480222336
• NII論文ID: 10027532656
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80183
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 9696880
• Web Site: http://id.ndl.go.jp/bib/9696880
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9696880
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80183
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可