A Novel Pair of Terminal Protein and Telomere-Associated Protein for Replication of the Linear Chromosome of<i>Streptomyces griseus</i>IFO13350

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  • SUZUKI Hirokazu
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
  • MARUSHIMA Kazuya
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
  • OHNISHI Yasuo
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo
  • HORINOUCHI Sueharu
    Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo

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  • A Novel Pair of Terminal Protein and Telomere-Associated Protein for Replication of the Linear Chromosome of Streptomyces griseus IFO13350

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The linear chromosome of Streptomyces griseus IFO13350 contains not only atypical telomere sequences but also probable pseudogenes for two typical telomeric proteins. Two identical operons (SGR98t-SGR97t near one telomere and SGR7041t-SGR7042t near the other telomere) in the terminal inverted repeat sequence were predicted to encode a novel pair of telomeric proteins. SGR97t, a 185-amino-acid protein showing only 18% amino acid sequence identity to typical terminal proteins of Streptomyces, was found to be attached to the chromosomal ends, as determined by immunological analysis. On the other hand, electrophoretic mobility shift assays showed that SGR98t, an 837-amino-acid protein having a DnaB-like helicase C-terminal domain, was capable of binding specifically to the single-stranded terminal DNA corresponding to the 3′ overhang of the replication intermediate. These results indicate that SGR97t (and SGR7042t) and SGR98t (and SGR7041t) were the functional telomeric proteins in the replication of the linear chromosome of S. griseus IFO13350.

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