Cloning of the gene encoding α-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme
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- NOZAKI Hiroyuki
- AminoScience Laboratories, Ajinomoto Co., Inc.
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- KURODA Shinji
- AminoScience Laboratories, Ajinomoto Co., Inc.
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- WATANABE Kunihiko
- AminoScience Laboratories, Ajinomoto Co., Inc.
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- YOKOZEKI Kenzo
- AminoScience Laboratories, Ajinomoto Co., Inc.
書誌事項
- タイトル別名
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- Cloning of the Gene Encoding .ALPHA.-Methylserine Hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and Characterization of the Recombinant Enzyme
- Cloning of the gene encoding アルファ methylserine hydroxymethyltransferase from Aminobacter sp AJ110403 and Ensifer sp AJ110404 and characterization of the recombinant enzyme
- Cloning of the Gene Encoding α-Methylserine Hydroxymethyltransferase from<i>Aminobacter</i>sp. AJ110403 and<i>Ensifer</i>sp. AJ110404 and Characterization of the Recombinant Enzyme
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抄録
Genes encoding α-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5′-phosphate. The Vmax of these enzymes catalyzing the conversion of α-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404).
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (11), 3002-3005, 2008
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681455945088
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- NII論文ID
- 10027534279
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 9724057
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可