Proteomics Profiling of Pituitary, Adrenal Gland, and Splenic Lymphocytes in Rats with Middle Cerebral Artery Occlusion

• XIONG Xingui

  Institute of Integrated Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University

• LIANG Qinghua

  Institute of Integrated Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University

• CHEN Jiang

  Center of Telemedicine, Xiangya Hospital, Central South University

• FAN Rong

  Institute of Integrated Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University

• CHENG Tianli

  Institute of Integrated Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University

抄録

Ischemic strokes are often accompanied by serious brain injury and poor prognosis, but the molecular mechanisms of primary and secondary injury have not been fully understood. The aim of the present study was to investigate the protein profile in the rat pituitary, adrenal gland, and splenic lymphocyte using proteomics techniques, and to elucidate potential changes in the immune neuroendocrine system following cerebral ischemia injury in rats. Out of the 41 differentially expressed protein spots identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-MS-TOF), 13 proteins were closely related to the immune and/or the neuroendocrine system, and the other proteins might have different functions through other mechanisms in middle cerebral artery occlusion (MCAO) rats. The results showed that (i) the immune neuroendocrine system was obviously changed, and the changes might be important pathological mechanisms in brain injury after cerebral ischemia, and (ii) ischemic brain damage is co-regulated by several mechanisms. The results might lay the foundations for further research on pathological mechanisms in cerebral ischemia.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (3), 657-664, 2009

  公益社団法人 日本農芸化学会

キーワード

• cerebral ischemia
• neuroendocrine-immune system
• proteomics
• mass spectrometry

詳細情報

• CRID: 1390001206479896960
• NII論文ID: 10027538796
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80717
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10199181
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10199181
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80717
• Web Site: https://search.jamas.or.jp/link/ui/2009251779
• 本文言語コード: en
• データソース種別:
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