Discrimination of Aliphatic Substrates by a Single Amino Acid Substitution in Bacillus badius and Bacillus sphaericus Phenylalanine Dehydrogenases

• TACHIBANA Shinjiro

  Biotechnology Research Center, Toyama Prefectural University

• KUWAMORI Yuko

  Biotechnology Research Center, Toyama Prefectural University

• ASANO Yasuhisa

  Biotechnology Research Center, Toyama Prefectural University

Bibliographic Information

Other Title

• Discrimination of Aliphatic Substrates by a Single Amino Acid Substitution in<i>Bacillus badius</i>and<i>Bacillus sphaericus</i>Phenylalanine Dehydrogenases

Abstract

Replacement of glycine by serine at positions 123 and 124 of phenylalanine dehydrogenases from Bacillus badius and Bacillus sphaericus respectively strikingly decreased enzyme activity toward aromatic amino acids and resulted in an elevation of relative activity toward aliphatic amino acids. The mutant from B. badius preferentially dehydrogenated branched-chain amino acids, while that from B. sphaericus acted on amino acids with straight-chain amino acids.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 73 (3), 729-732, 2009

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• phenylalanine dehydrogenase
• site-directed mutagenesis
• <I>Bacillus badius</I>
• <I>Bacillus sphaericus</I>

Details

• CRID: 1390001206480018304
• NII Article ID: 10027539133
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.80626
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 10199323
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10199323
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80626
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed