Development of a Homologous Transformation System for<i>Aspergillus aculeatus</i>Based on the<i>sC</i>Gene Encoding ATP-Sulfurylase

• ADACHI Hiromi

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• TANI Shuji

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• KANAMASA Shin

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• SUMITANI Jun-ichi

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• KAWAGUCHI Takashi

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

書誌事項

タイトル別名

• Development of a Homologous Transformation System for Aspergillus aculeatus Based on the sC Gene Encoding ATP-Sulfurylase

抄録

A homologous transformation system was developed using the endogenous ATP-sulfurylase gene, AasC, as a selectable marker in Aspergillus aculeatus. Spontaneous mutation was proved to be beneficial in isolating AasC-deficient mutants. Molecular analysis of sC⁺ transformants revealed that the frequency of single copy integration at ATP-sulfurylase locus was more than 40%.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (5), 1197-1199, 2009

  公益社団法人 日本農芸化学会

キーワード

• <I>Aspergillus aculeatus</I>
• ATP-sulfurylase gene
• homologous transformation
• two-way selection

詳細情報

• CRID: 1390282681453703936
• NII論文ID: 10027541507
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.80772
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10235250
• Web Site: http://id.ndl.go.jp/bib/10235250
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10235250
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.80772
• 本文言語コード: en
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