Identification of<i>sam4</i>as a<i>rad24</i>Allele in<i>Schizosaccharomyces pombe</i>

• OOWATARI Yasuo

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• TOMA Kenichi

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• OZOE Fumiyo

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• KAWAMUKAI Makoto

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

書誌事項

タイトル別名

• Identification of sam4 as a rad24 Allele in Schizosaccharomyces pombe

抄録

Fission yeast requires nutritional starvation to switch the mitotic cell cycle to sexual differentiation, but sam mutants, of which we had isolated nine alleles, mate without the starvation condition. These mutants are useful for understanding the mechanism underlying the way cells sense nutritional starvation and change the cell cycle. To identify the sam allele, we first sought phenotypes other than the original sam phenotype. We found that all nine sam mutants were sensitive to 1 M KCl, that sam2, sam3, sam4 and sam9 were sensitive to 0.1 M CaCl₂, and that only the sam4 mutant was sensitive to 150 J/m² UV. This peculiar phenotype of sam4 suggested to us that sam4 might be an allele of rad24, which encodes a 14-3-3 protein. In fact, the Rad24 protein disappeared in sam4 and the rad24 mRNA was not transcribed in sam4. In addition, the mutation that changed Gln to a stop codon was found in the rad24 locus of sam4. Hence we concluded that sam4 is an allele of rad24. We also found that over-expression of rad24 or rad25 (a paralog of rad24) has a suppressive effect on sam1, and that sam1 was not an allele of rad24 nor rad25. Thus 14-3-3 proteins are deeply involved in the switching of the mitotic cell cycle to the sexual differentiation of fission yeast.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (7), 1591-1598, 2009

  公益社団法人 日本農芸化学会

キーワード

• <I>Schizosaccharomyces pombe</I>
• fission yeast
• meiosis
• sexual differentiation

詳細情報

• CRID: 1390282681455987072
• NII論文ID: 10027543618
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.90103
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10303665
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10303665
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90103
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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