Tumor-suppressive lipoxygenases inhibit the expression of c-myc mRNA coding region determinant-binding protein/insulin-like growth factor 2 mRNA-binding protein 1 in human prostate carcinoma PC-3 cells

• KAWAKAMI Yuki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• KUBOTA Noriyuki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• EKUNI Natsuki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• SUZUKI-YAMAMOTO Toshiko

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• KIMOTO Masumi

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• YAMASHITA Hiromi

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• TSUJI Hideaki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• YOSHIMOTO Tanihiro

  Department of Molecular Pharmacology, Kanazawa University Graduate School of Medical Science

• JISAKA Mitsuo

  Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University

• TANAKA Junko

  Department of Molecular Pharmacology, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• FUJIMURA Hirofumi F.

  Department of Molecular Pharmacology, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• MIWA Yoshihiro

  Department of Molecular Pharmacology, Graduate School of Comprehensive Human Sciences, University of Tsukuba

• TAKAHASHI Yoshitaka

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

書誌事項

タイトル別名

• Tumor-Suppressive Lipoxygenases Inhibit the Expression of c-myc mRNA Coding Region Determinant-Binding Protein/Insulin-Like Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells
• Tumor-Suppressive Lipoxygenases Inhibit the Expression of c-<i>myc</i>mRNA Coding Region Determinant-Binding Protein/Insulin-Like Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells

抄録

8S-Lipoxygenase (8S-LOX) is known as a mouse homolog of human 15S-LOX-2. 15S-LOX-2 was down-regulated in malignant transformation of prostate epithelial cells, and its overexpression caused cell cycle arrest. To determine whether 8S-LOX would have a growth inhibitory effect on prostate carcinoma, we obtained human prostate carcinoma PC-3 cells expressing 8S-LOX or 15S-LOX-2. The growth rate of cells measured by colorimetric assay was reduced by expression of 8S-LOX and 15S-LOX-2. The addition to enzyme-expressing cells of arachidonic acid enhanced the growth suppressive effect, whereas the expression of catalytically inactive mutants did not affect cell growth, suggesting that the effect was product-dependent. DNA microarray and quantitative reverse transcription-PCR analyses revealed that the c-myc mRNA coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein 1 (CRD-BP/IMP-1), known as an oncofetal protein, was down-regulated in 8S-LOX- and 15S-LOX-2-expressing PC-3 cells. Targeted knockdown of CRD-BP/IMP-1 resulted in inhibition of the DNA synthesis rate of PC-3 cells as measured by bromodeoxyuridine incorporation. We propose that expression of 8S-LOX and 15S-LOX-2 suppresses CRD-BP/IMP-1 expression, resulting in inhibition of human prostate carcinoma PC-3 cell proliferation.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (8), 1811-1817, 2009

  公益社団法人 日本農芸化学会

キーワード

• c-<I>myc</I> mRNA coding region determinant-binding protein/insulin-like growth factor II mRNA-binding protein 1 (CRD-BP/IMP-1)
• human prostate carcinoma PC-3 cells
• 8<I>S</I>-lipoxygenase
• 15<I>S</I>-lipoxygenase-2

詳細情報

• CRID: 1390282681455680000
• NII論文ID: 10027544597
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.90185
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10338263
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10338263
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90185
• Web Site: https://search.jamas.or.jp/link/ui/2010027966
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可