Improvement of heterologous protein production in Aspergillus oryzae by RNA interference with α-amylase genes
-
- NEMOTO Takashi
- Department of Biotechnology, The University of Tokyo
-
- MARUYAMA Jun-ichi
- Department of Biotechnology, The University of Tokyo
-
- KITAMOTO Katsuhiko
- Department of Biotechnology, The University of Tokyo
書誌事項
- タイトル別名
-
- Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with .ALPHA.-Amylase Genes
- Improvement of heterologous protein production in Aspergillus oryzae by RNA interference with a amylase genes
- Improvement of Heterologous Protein Production in<i>Aspergillus oryzae</i>by RNA Interference with α-Amylase Genes
この論文をさがす
抄録
Aspergillus oryzae RIB40 has three α-amylase genes (amyA, amyB, and amyC), and secretes α-amylase abundantly. However, large amounts of endogenous secretory proteins such as α-amylase can compete with heterologous protein in the secretory pathway and decrease its production yields. In this study, we examined the effects of suppression of α-amylase on heterologous protein production in A. oryzae, using the bovine chymosin (CHY) as a reporter heterologous protein. The three α-amylase genes in A. oryzae have nearly identical DNA sequences from those promoters to the coding regions. Hence we performed silencing of α-amylase genes by RNA interference (RNAi) in the A. oryzae CHY producing strain. The silenced strains exhibited a reduction in α-amylase activity and an increase in CHY production in the culture medium. This result suggests that suppression of α-amylase is effective in heterologous protein production in A. oryzae.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 73 (11), 2370-2373, 2009
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206480637696
-
- NII論文ID
- 10027547386
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 10458732
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可