Hamartin-Hsp70 Interaction Is Necessary for Akt-Dependent Tuberin Phosphorylation during Heat Shock

• INOUE Hirohumi

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• NDONG Moussa

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• SUZUKI Tsukasa

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• KAZAMI Machiko

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• UYAMA Takumi

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• KOBAYASHI Ken-Ichi

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• TADOKORO Tadahiro

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

• YAMAMOTO Yuji

  Department of Applied Biology and Chemistry, Tokyo University of Agriculture

抄録

Hamartin and tuberin interact directly to regulate cell growth negatively. In this study, far-western blotting revealed that hamartin binds directly Heat shock protein 70 (Hsp70), even in the absence of tuberin. While the hamartin-tuberin complex acts as a sensor for a variety of types of stress, it is unclear how the complex is regulated under stress conditions. We found that the hamartin-Hsp70 interaction is stabilized during heat shock. On the other hand, tuberin underwent degradation through phosphorylation in an Akt-dependent manner. Furthermore, we found that when Hsp70 expression was inhibited by N-formyl-3,4-methylenedioxy-benzylidene-γ-butyrolactam (KNK437), Akt phosphorylation on site Ser308 diminished and tuberin was not phosphorylated at Thr1462 during heat shock. We conclude that both hamartin and Hsp70 increase in response to heat shock, whereas tuberin is phosphorylated and thereafter degraded via the PI3K/Akt pathway. Through this pathway, hamartin-Hsp70 plays a crucial role as a scaffolding protein that transfers the Akt signal to tuberin.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (11), 2488-2493, 2009

  公益社団法人 日本農芸化学会

キーワード

• hamartin
• tuberin
• Heat shock protein 70 (Hsp70)
• phosphorylation
• Akt

詳細情報

• CRID: 1390282681457336960
• NII論文ID: 10027548025
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.90489
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10459426
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10459426
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90489
• 本文言語コード: en
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