Purification and Biochemical Characterization of Recombinant Rice UDP-Arabinopyranose Mutase Generated in Insect Cells

Abstract

Plants utilize UDP-arabinofuranose (UDP-Araf) in the biosynthesis of Araf-containing complex carbohydrates. UDP-Araf is synthesized from UDP-arabinopyranose by UDP-arabinopyranose mutases (UAMs). Here we describe the heterologous expression of rice (Oryza sativa) UAM genes in insect cells and report some of their enzymatic properties. Recombinant UAMs might serve as useful tools for the biosynthesis of UDP-Araf and might be better than chemical synthesis.

Journal

Bioscience, Biotechnology, and Biochemistry  

Bioscience, Biotechnology, and Biochemistry 74(1), 191-194, 2010-01-23 

Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  7

You must have a user ID to see the references.If you already have a user ID, please click "Login" to access the info.New users can click "Sign Up" to register for an user ID.

Codes

  • NII Article ID (NAID) :
    10027550650
  • NII NACSIS-CAT ID (NCID) :
    AA10824164
  • Text Lang :
    ENG
  • Article Type :
    NOT
  • ISSN :
    09168451
  • NDL Article ID :
    10533150
  • NDL Source Classification :
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No. :
    Z53-G223
  • Databases :
    CJP  NDL  J-STAGE 

Export