An AP-1-Like Transcription Factor, NAP-1, Regulates Expression of the Glutathione<i>S</i>-Transferase and NADH:Flavin Oxidoreductase Genes in<i>Neurospora crassa</i>
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- TAKAHASHI Masakazu
- Faculty of Life Sciences, Toyo University
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- YAMASHITA Kazuhiro
- Faculty of Life Sciences, Toyo University
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- SHIOZAWA Azusa
- Faculty of Life Sciences, Toyo University
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- ICHIISHI Akihiko
- Faculty of Life Sciences, Toyo University
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- FUKUMORI Fumiyasu
- Faculty of Life Sciences, Toyo University
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- FUJIMURA Makoto
- Faculty of Life Sciences, Toyo University
書誌事項
- タイトル別名
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- An AP-1-Like Transcription Factor, NAP-1, Regulates Expression of the Glutathione S-Transferase and NADH:Flavin Oxidoreductase Genes in Neurospora crassa
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AP-1-like transcription factors play crucial roles in oxidative stress responses in yeast and filamentous fungi. The deletion of an AP-1-like transcription factor gene, nap-1, in Neurospora crassa slightly increased its sensitivity to oxidative stressors, including menadione. Microarray and quantitative real-time reverse transcriptase-PCR analyses were employed to identify menadione-inducible genes (migs) and the roles of NAP-1 in their regulation. N. crassa migs include three putative glutathione S-transferase genes and two NADH:flavin oxidoreductase genes, orthologs of OYE2 and OYE3, both of which play roles in menadione tolerance in Saccharomyces cerevisiae. Menadione induced nuclear localization of NAP-1, and oxidative upregulation of many of migs were NAP-1 dependent. Genes for a thioredoxin, a glutathione reductase, and a glutathione peroxidase were slightly upregulated by the chemical only in the wild-type strain, suggesting that NAP-1 is involved in their oxidative induction and probably dose not contribute to high-level constitutive expressions of such genes.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (4), 746-752, 2010
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206478807552
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- NII論文ID
- 10027553641
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10656424
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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