The Unique Kinetic Behavior of the Very Large NAD-Dependent Glutamate Dehydrogenase from<i>Janthinobacterium lividum</i>

• KAWAKAMI Ryushi

  Analytical Research Center for Experimental Sciences, Saga University

• OYAMA Masaki

  Department of Applied Biochemistry and Food Science, Faculty of Agriculture, Saga University

• SAKURABA Haruhiko

  Department of Applied Biological Science, Faculty of Agriculture, Kagawa University

• OHSHIMA Toshihisa

  Microbial Genetics Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University

書誌事項

タイトル別名

• The Unique Kinetic Behavior of the Very Large NAD-Dependent Glutamate Dehydrogenase from Janthinobacterium lividum

抄録

The kinetics of a very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum showed positive cooperativity toward α-ketoglutarate and NADH, and the Michaelis-Menten type toward ammonium chloride in the absence of the catalytic activator, L-aspartate. An increase in the maximum activity accompanied the decrease in the S_0.5 values for α-ketoglutarate and NADH with the addition of L-aspartate, and the kinetic response for α-ketoglutarate changed completely to a typical Michaelis-Menten type in the presence of 10 mM L-aspartate.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (4), 884-887, 2010

  公益社団法人 日本農芸化学会

キーワード

• very large glutamate dehydrogenase
• kinetic analysis
• reductive amination
• activation
• positive cooperativity

詳細情報

• CRID: 1390001206478731904
• NII論文ID: 10027554364
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.90925
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10656702
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10656702
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90925
• 本文言語コード: en
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• 抄録ライセンスフラグ: 使用不可