Molecular Cloning, Characterization, and Differential Expression of a Lanosterol Synthase Gene from<i>Ganoderma lucidum</i>

• SHANG Chang-Hua

  College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture

• SHI Liang

  College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture

• REN Ang

  College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture

• QIN Lei

  College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture

• ZHAO Ming-Wen

  College of Life Sciences, Nanjing Agricultural University, Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture

書誌事項

タイトル別名

• Molecular Cloning, Characterization, and Differential Expression of a Lanosterol Synthase Gene from Ganoderma lucidum

抄録

A homology-based PCR method was used to clone a cDNA encoding lanosterol synthase (LS) from Ganoderma lucidum (G. lucidum), which produces triterpenes. The cDNA of the LS (GenBank accession no. GQ169528) was found to contain an open reading frame (ORF) of 2,181 bp encoding a 726 amino acid polypeptide, whereas the LS genomic DNA sequence (GenBank accession no. GQ169529) consists of 2,924 bp. Functional complementation of G. lucidum LS (Gl-LS) in an erg7 yeast strain lacking LS activity demonstrated that the cloned cDNA encoded a functional LS. Analysis of the Gl-LS transcript profiles revealed a positive correlation between the pattern of LS gene expression and triterpenes content changes in G. lucidum during development. Up-regulation of expression of the Gl-LS gene by methyl jasmonate (MeJA) in the mycelia was also demonstrated by real-time RT-PCR. Up-regulation of the Gl-LS promoter activity by MeJA was also investigated.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (5), 974-978, 2010

  公益社団法人 日本農芸化学会

キーワード

• gene cloning
• <I>Ganoderma lucidum</I>
• lanosterol synthase
• differential expression
• triterpenes biosynthesis

詳細情報

• CRID: 1390001206477773184
• NII論文ID: 10027554926
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.90833
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10688794
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10688794
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.90833
• 本文言語コード: en
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