Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System

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  • WU Chi-Ming
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
  • HSUAN Shih-Ling
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
  • CHEN Zeng-Weng
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University
  • JINN Tzyy-Rong
    Graduate Institute of Chinese Medical Science, College of Chinese Medicine, China Medical University
  • HUANG Chienjin
    Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University
  • LIAO Jiunn-Wang
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
  • CHEN Ter-Hsin
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University
  • LIAO Chih-Ming
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
  • LEE Wei-Cheng
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
  • WU Tzong-Yuan
    Department of Bioscience Technology, Chung Yuan Christian University
  • CHIEN Maw-Sheng
    Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University

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抄録

To develop an economical, easy technique for producing recombinant E2 glycoprotein (rE2) of classical swine fever virus (CSFV) as a candidate immunogen, a bi-cistronic baculovirus/larvae expression vector was constructed using p10 promoter, an internal ribosome entry site, and the gfp gene. Trichoplusia ni larvae were successfully infected with the occluded recombinant baculovirus via feed, and the characteristics of rE2 were confirmed by immunoblot and glycosylation stain. rE2 at a concentration of 0.6–0.8 mg/ml without degradation was obtained from hemolymphs of infected larvae that emitted high levels of green fluorescence. Immunization assays indicated that mice and piglets immunized with rE2-containing hemolymph elicited high titers of anti-CSFV E2 antibodies with virus-neutralizing activity. This is the first study to indicate that baculovirus/T. ni larvae-expressed rE2 can be served as a vaccine candidate. This system provides an economical alternative for the production of vaccine components in the veterinary industry.

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