Characterization of Juvenile Hormone Epoxide Hydrolase and Related Genes in the Larval Development of the Silkworm<i>Bombyx mori</i>

• SEINO Atsushi

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• OGURA Takehiko

  Department of Applied Life Sciences, Kyoto University

• TSUBOTA Takuya

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• SHIMOMURA Masaru

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• NAKAKURA Takayo

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• TAN Anjiang

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• MITA Kazuei

  Insect Genome Research Unit, National Institute of Agrobiological Science

• SHINODA Tetsuro

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

• NAKAGAWA Yoshiaki

  Department of Applied Life Sciences, Kyoto University

• SHIOTSUKI Takahiro

  Invertebrate Gene Function Research Unit, National Institute of Agrobiological Science

書誌事項

タイトル別名

• Characterization of Juvenile Hormone Epoxide Hydrolase and Related Genes in the Larval Development of the Silkworm Bombyx mori

抄録

Juvenile hormone epoxide hydrolases (JHEHs) are a family of enzymes that hydrolyze juvenile hormones (JHs). They are important in terms of organ-specific regulation and irreversible degradation. In contrast to three JHEH genes (jheh) in Drosophila melanogaster and five jheh in Tribolium castaneum, only one jheh gene has been reported to date in lepidopteran insects. By searching a genome database of the silkworm, KAIKOBLAST, five JHEH-related genes (jheh-r), in addition to Bmjheh, were found. Developmental changes in mRNA expression were brought about revealing several unique patterns for each of jheh-r as to developmental stages and organ-specificity. Recombinant proteins of JHEH-r were expressed using a baculovirus system to evaluate their enzymatic activities. Three of the five JHEH-r recombinant proteins had JH hydrolytic activities. This is the first report on lepidopteran jheh-related genes and also provides the comprehensive analysis of multiple jheh-related genes in an insect species with respect to their functions in enzyme activities.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 74 (7), 1421-1429, 2010

  公益社団法人 日本農芸化学会

キーワード

• juvenile hormone epoxide hydrolase
• silkworm
• <I>Bombyx mori</I>
• cDNA cloning
• juvenile hormone (JH) titer regulation

詳細情報

• CRID: 1390282681456157312
• NII論文ID: 10027557293
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.100104
• COI: 1:STN:280:DC%2BC3cjgvFagtA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 10766605
• PubMed: 20622465
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10766605
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.100104
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可