Ultrastructural Changes in Granulosa Cells in Porcine Antral Follicles Undergoing Atresia Indicate Apoptotic Cell Death.

DOI Web Site Web Site 14 Citations 39 References
  • SUGIMOTO Miki
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
  • MANABE Noboru
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
  • KIMURA Yoshihiro
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
  • MYOUMOTO Akira
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
  • IMAI Yuzuru
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
  • OHNO Hiroko
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan
  • MIYAMOTO Hajime
    Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University, Kyoto 606-8502, Japan

Bibliographic Information

Other Title
  • Ultrastructural Changes in Granulosa Ce

Search this article

Abstract

Ultrastructural changes in follicular granulosa cells during atresia were examined in pig ovaries. No apoptotic cells were observed in healthy follicles. However, in follicles in the early stage of atresia apoptosis occurred in granulosa cells located on the inner surface of the follicular wall. In these apoptotic granulosa cells, nuclear condensation with large clumps of chromatin and cytoplasmic condensation but with retention of the integrity of organelles were observed by electron microscopy. As atresia progressed, pyknotic granulosa cells and apoptotic bodies increased in number in the follicular wall. In follicles in the late stage of atresia, apoptotic bodies were suspended in the follicular antrum. Concurrently, cell debris without distinct membranes but with condensed chromatin, degenerated apoptotic bodies, occurred in follicles. The apoptotic bodies and cell debris were occasionally surrounded and phagocytosed by neighboring granulosa cells. In follicles in the advanced stage of atresia, apoptotic bodies and cell debris were fed by macrophages.

Journal

Citations (14)*help

See more

References(39)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top