IVF、ICSIに由来するマウス初期胚および産仔のSCE発生頻度
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- Shimoi Gaku
- Faculty of Bioindustry, Tokyo University of Agriculture
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- Kameyama Yuichi
- Faculty of Bioindustry, Tokyo University of Agriculture
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- Kudoh Kenichi
- School of Veterinary Medicine, Kitasato University
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- Hashizume Ryoichi
- Faculty of Bioindustry, Tokyo University of Agriculture
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- Ito Masao
- Faculty of Bioindustry, Tokyo University of Agriculture
書誌事項
- タイトル別名
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- Sister Chromatid Exchange Frequency in Early Embryos and Offspring Derived from IVF or ICSI in Mice
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抄録
To demonstrate chromosomal injuries associated with in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI), sister chromatid exchange (SCE), an indicator of DNA damage, was investigated and the cytogenetic influence of embryonic manipulation on chromosomes of early embryos and offspring was investigated. SCE analysis was performed in early embryos and offspring obtained by IVF, ICSI, or in vivo fertilization (control). To label chromosomes of early embryos, the embryos were cultured in BrdU-supplemented medium for 2 cell cycles. For offspring, BrdU solution was repeatedly injected intraperitoneally. Chromosome samples prepared from the embryos and the bone marrow cells of offspring were stained by the Fluorescence plus Giemsa (FPG) method. In the IVF and ICSI groups, the rates of early development, implantation and offspring were lower than those in the control group. The SCE frequency of early embryos was significantly higher in the IVF and ICSI groups than in the control group (P < 0.05). In contrast, the SCE frequency of offspring in the IVF and ICSI groups was not significantly different from that in the control group. These findings suggest that embryos having serious DNA damage due to embryonic manipulation may be eliminated in a relatively early developmental step and may not reach term.<br>
収録刊行物
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- 日本哺乳動物卵子学会誌
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日本哺乳動物卵子学会誌 27 (3), 114-121, 2010
日本卵子学会
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詳細情報 詳細情報について
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- CRID
- 1390282679314702848
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- NII論文ID
- 10027595031
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- NII書誌ID
- AN10548943
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- ISSN
- 13475878
- 13417738
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- NDL書誌ID
- 10894191
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可