Agrobacterium tumefaciens-mediated transformation of the violet root-rot fungus, Helicobasidium mompa, and the effect of activated carbon

DOI Web Site Web Site 被引用文献2件 参考文献26件

書誌事項

タイトル別名
  • <i>Agrobacterium tumefaciens</i>-mediated transformation of the violet root-rot fungus, <i>Helicobasidium mompa</i>, and the effect of activated carbon

この論文をさがす

抄録

<p>Agrobacterium tumefaciens-mediated transformation (ATMT) has been successfully applied to the violet root-rot fungus Helicobasidium mompa, which is the causal agent of violet root-rot disease. The A. tumefaciens strains carried a binary plasmid vector containing the hygromycin B phosphotransferase gene (hph) controlled by the heterologous fungal Agaricus bisporus P-gpd (glyceraldehyde-3-phosphate dehydrogenase) promoter and the trpC terminator. The transformation system was optimized using defined cocultivation conditions. When H. mompa strain V17 was cocultivated with A. tumefaciens strain AGL-1 using 5% agar, we obtained more hygromycin-resistant colonies than with strains EHA105 or MAFF301222 using 2% agar. In addition, our results suggest that the activated carbon is necessary in ATMT to reduce background growth of H. mompa. The presence of the hph gene in transformants was detected by polymerase chain reaction (PCR), and single-copy integration of the marker gene was demonstrated by Southern blot analysis. Thus, the ATMT system can be considered a promising tool for insertional mutagenesis studies of H. mompa</p>

収録刊行物

  • Mycoscience

    Mycoscience 52 (1), 24-30, 2011

    一般社団法人 日本菌学会

被引用文献 (2)*注記

もっと見る

参考文献 (26)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ