In vitro processing of the dsRNase precursor isolated from silkworm midgut tissue, Bombyx mori

• Arimatsu Yuji

  Center for Bioresource Field Science, Kyoto Institute of Technology

• Sugimura Yukio

  Graduate School of Science and Technology, Kyoto Institute of Technology

• Furusawa Toshiharu

  Center for Bioresource Field Science, Kyoto Institute of Technology

抄録

Analysis of cDNA encoding the double-stranded ribonuclease (Bm-dsRNase) of the silkworm, Bombyx mori, elucidated a possible conclusion that the 41kDa mature dsRNase is produced from the 45kDa precursor protein in the middle midgut tissue by post-translational processing. To strengthen this speculation, the 41kDa protein was produced in vitro: the 43kDa-45kDa protein-rich fraction was partially purified by gel filtration and then treated with bovine trypsin. Specific conversion of the 43kDa and 45kDa proteins into the enzymically-active 41kDa protein was demonstrated by a Western blot analysis and dsRNase activity assay, strongly supporting that the 41kDa Bm-dsRNase is produced in vivo by the limited proteolysis that was induced by an endogenous trypsin-like enzyme, and immediately released into midgut lumen.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｉｎｓｅｃｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ　ａｎｄ　Ｓｅｒｉｃｏｌｏｇｙ 79 (3), 3_125-3_127, 2010

  社団法人 日本蚕糸学会

キーワード

• <i>Bombyx mori</i>
• dsRNase
• precursor protein
• post-translational processing

詳細情報

• CRID: 1390282680172162688
• NII論文ID: 130004464173
• NII論文ID: 10027849672
• NII書誌ID: AA11558849
• DOI: 10.11416/jibs.79.3_125
• ISSN: 18847978
• ISSN: 13468073
• NDL書誌ID: 10979877
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10979877
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可