Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo

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  • HASUWA Hidetoshi
    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
  • MURO Yuko
    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
  • IKAWA Masahito
    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
  • KATO Noriko
    Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School JCL Bioassay Corp.
  • TSUJIMOTO Yoshihide
    Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School
  • OKABE Masaru
    Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University

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In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 59 (1), 105-107, 2010

    公益社団法人 日本実験動物学会

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