Cloning and Transcriptional Analysis of the Gene Encoding 5-Aminolevulinic Acid Synthase of the White-Rot Fungus<i>Phanerochaete sordida</i>YK-624

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  • MISUMI Kenta
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
  • SUGIURA Tatsuki
    Department of Bioscience, Graduate School of Science and Technology, Shizuoka University
  • YAMAGUCHI Shinya
    Department of Environment and Forest Resources Science, Faculty of Agriculture, Shizuoka University
  • MORI Toshio
    Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University
  • KAMEI Ichiro
    Faculty of Agriculture, University of Miyazaki
  • HIRAI Hirofumi
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
  • KAWAGISHI Hirokazu
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University Department of Bioscience, Graduate School of Science and Technology, Shizuoka University
  • KONDO Ryuichiro
    Department of Agro-environmental Sciences, Faculty of Agriculture, Kyushu University

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  • Cloning and Transcriptional Analysis of the Gene Encoding 5-Aminolevulinic Acid Synthase of the White-Rot Fungus Phanerochaete sordida YK-624

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In this study, we cloned the gene encoding 5-aminolevulinic acid synthase (ALAS) from the hyper-lignin-degrading fungus Phanerochaete sordida YK-624. The deduced amino acid sequence showed highest identity (93.0%) to ALAS of P. chrysosporium. Expression of the gene encoding ALAS, which we named aas, corresponded temporally with the expression and activity of manganese peroxidase.

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