Bacillus thuringiensis cry toxins bound specifically to various proteins via domain 3, which had a galactose-binding domain-like fold
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- KITAMI Madoka
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- KADOTANI Tomoyuki
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- NAKANISHI Kazuko
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- ATSUMI Shogo
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- HIGURASHI Satoshi
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- ISHIZAKA Takahisa
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- WATANABE Ayako
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
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- SATO Ryoichi
- Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology
書誌事項
- タイトル別名
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- <I>Bacillus thuringiensis</I> Cry Toxins Bound Specifically to Various Proteins <I>via</I> Domain III, Which Had a Galactose-Binding Domain-Like Fold
- Bacillus thuringiensis Cry toxins bound specifically to various proteins via domain III, which had a galactose-binding domain-like fold
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抄録
Cry toxins have been reported to bind not only to receptors on insect cells but also to several unrelated proteins. In this study, we investigated the binding properties of Bacillus thuringiensis Cry toxins, focusing on domain III, a Cry toxin region with a structure that of the galactose-binding domain-like. Cry1Aa, Cry1Ac, and Cry8Ca specifically bound to several proteins unrelated to insect midgut cells. Cry1Aa binding to Cry toxin-binding proteins was inhibited by a monoclonal antibody, 2C2, indicating that Cry1Aa binds to these Cry toxin-binding proteins through domain III. Cry1Aa binding to Bombyx mori aminopeptidase N and other Cry toxin-binding proteins was inhibited by carbonic anhydrase, a Cry toxin-binding protein. The binding regions of carbonic anhydrase and Bombyx mori aminopeptidase N were narrowed to regions of less than 20 amino acids that did not have any similarity, suggesting that Cry toxin domain III has a binding pocket for multiple proteins.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (2), 305-312, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681455147520
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- NII論文ID
- 10027897803
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10993280
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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