Effect of Tris-HCl Buffer on DNA Adsorption by a Variety of Soil Constituents

• Saeki Kazutoshi

  Biotron Institute, Kyushu University

• Kunito Takashi

  Faculty of Science, Shinshu University

• Sakai Masao

  Faculty of Agriculture, Kagoshima University

抄録

We investigated the effect of tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer (pH 7.0) as a bulk solution on the adsorption of DNA by gibbsite, goethite, montmorillonite, kaolinite, synthetic and natural allophanes, two humic acids and two andosols. The natural allophane, gibbsite, kaolinite and an andosol adsorbed significantly more DNA in a 0.1 M Tris-HCl buffer than in a 0.1 M NaCl solution (t-test, P<0.005). In contrast, montmorillonite adsorbed significantly less DNA in the Tris-HCl than NaCl solution (P<0.05). Care should be taken when using Tris-HCl in studies on the adsorption of extracellular DNA molecules by soil particles.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 26 (1), 88-91, 2011

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• DNA adsorption
• Tris-HCl buffer
• allophane
• soil
• montmorillonite

詳細情報

• CRID: 1390282679321873408
• NII論文ID: 10028099122
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me10172
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 10991437
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10991437
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/26/1/26_ME10172/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可